- Date: –09:45
- Location: On zoom : https://uu-se.zoom.us/j/68209194308
- Lecturer: Sebastian Lungu-Mitea, PhD, Drug Safety and Toxicology, Department of Pharmaceutical Biosciences, Uppsala University
- Contact person: Anna Nilsson
DE NOVO ESTABLISHMENT OF IN VITRO FISH BIOASSAYS VIA CRISPR/CAS-MEDIATED REPORTER GENE KNOCK-INS FOR PRO- AND RETROSPECTIVE ENVIRONMENTAL TOXICITY TESTING
Human toxicology has already undergone a paradigm shift in testing, commonly known as “toxicology in the 21st century”. It was plausible to replace rodent-based in vivo tests with human-based in vitro methods, given that the differences between species introduced substantial bias. However, in environmental toxicology, where the protection goal is primarily aquatic species, toxicity testing is still dominated by in vivo methods, leading to several ethical, economic, and technical issues regarding high-throughput screening. This project aims to design novel reporter gene assays in zebrafish cell lines via the CRISPR/Cas endonuclease technology. The latter will be used to assess pollutants and environmental samples in both prospective and retrospective toxicology and try to overcome current methodological pitfalls.