NCMSI - MALDI-MSI Instruments
The NCMSI platform is one of the largest and most modern MALDI-MSI laboratories in Europe. It has two MALDI TOF-TOF mass spectrometers (Ultraflextreme, Bruker Daltonics; installed in 2012 and 2014) and two MALDI-q-TOF instruments – a Synapt G2-S HDMS with ion mobility separation (Waters Corp., installed in 2015) and a Maxis Impact mass spectrometer (Bruker Daltonics, installed in 2012). The laboratory also has several MALDI matrix application instruments including two ImagePrep instruments (Bruker Daltonics; uses vibrational vaporization technology), one CHIP-1000 Chemical Printer (Shimadzu; uses piezoelectric spotting technology), and one TM-Sprayer Tissue MALDI Sample Prep System (HTX Technologies; uses heated spray technology).
MALDI-TOF-TOF ultrafleXtreme, Bruker Daltonics
The ultrafleXtreme instruments can perform 2 kHz speed in MS mode and 1 kHz in MS/MS mode with ultrahigh performance and flexibility for a broad variety of MALDI-MSI applications. Can analyze samples at near cellular spatial resolution (10-20µm). Requires tissue sections to be placed on conductive glass slides. Instruments installed 2012 and 2014.
MALDI/ESI Q-Tof Synapt G2si, Waters Corp.
The MALDI SYNAPT G2-Si is powered by a solid-state laser with a repetition rate of 2.5 kHz to maximize the speed of the analysis. In addition, SYNAPT is well suited for analysis of tissue sections placed on regular microscope glass slides (non-conductive). Spatial resolution of 50-10µm. Instrument installed 2015.
SI Q-Tof Maxis Impact, Bruker Daltonics
The instrument can produce >50,000 full-sensitivity resolution. It has high dynamic range and five orders of magnitude is achieved at UHPLC speeds from 50 GBit/sec using sampling technology. Instrument installed 2012.
ESI LTQ (ion trap) MS, ThermoScientific Corp.
This instrument has MSn performance. It was installed 2004
Matrix Application and Tissue Cutting Instruments
The TM-Sprayer provides heat to the matrix solution to accelerate adsorption into the tissue, as well as a controlled flow of dry air or nitrogen to focus the spray and control drying time.
ImagePrep, Bruker Daltonics
Using vibrational vaporization technology, ImagePrep provides reproducible matrix preparations for MALDI-MSI with a fully automated process
CHIP-1000 Chemical Printer, Shimadzu Corp.
This instrument delivers picoliter volumes of reagent to a tissue section surface using piezoelectric technology. To support the visualization of the droplet and the area of interest, the system has a camera and an in built scanner.
Leica CM1900 Cryostat, Leica Microsystems
The Leica CM1900 instrument is a rapid sectioning cryostat for advanced cutting of small animal organs.